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Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles

Received: 31 May 2022     Accepted: 15 June 2022     Published: 27 June 2022
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Abstract

Gold nanoparticles (AuNPs) have been widely studied in a great mass of cellular biomarkers detection and diagnostics due to their unique combination of physical and optical properties. Sea urchin-like gold nanoparticles (SUL-AuNPs) are well characterized multi-thorn gold nanostructures which possess at least four gold nanothorns on spherical gold surface, mimicking the morphology of sea urchin. Compared to spherical AuNPs, SUL-AuNPs showed a wide variety of light absorption and scattering properties, and the Surface-enhanced Raman Scattering (SERS) properties of SUL-AuNPs were also widely studied dependent on their surface morphology. Herein, three different diameters of SUL-AuNPs based on spherical AuNPs seed-mediated growth method by altering the amount of AuNPs in reaction system had been synthesized. The UV-vis spectrum of synthesized SUL-AuNPs displayed a shift from 550 nm to 650 nm compared to spherical AuNPs. FRET method was applied for the detection of GSH in hepatocytes and cell extracts using rhodamine B (RB) functionalized SUL-AuNPs, among the synthesized three different diameters SUL-AuNPs, 100nm RB-SUL-AuNPs displayed highest sensitivity for GSH detection. What's more, all synthesized SUL-AuNPs turned out to be membrane-permeable, and displayed ignorable cytotoxicity, which make SUL-AuNPs promising cellular thiols detection probes. In particular, it should be noted that the application of RB functionalized SUL-AuNPs exemplify ongoing efforts in design and utility of multifunctional nanoplatforms of SUL-AuNPs.

Published in International Journal of Biomedical Science and Engineering (Volume 10, Issue 2)
DOI 10.11648/j.ijbse.20221002.14
Page(s) 54-60
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

FRET, Glutathione, Sea Urchin-Like Gold Nanoparticle, Thiol Detection

References
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Cite This Article
  • APA Style

    Ting Lei Zhao, Zhe Zhang, Dan Li, Yanxialei Jiang. (2022). Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles. International Journal of Biomedical Science and Engineering, 10(2), 54-60. https://doi.org/10.11648/j.ijbse.20221002.14

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    ACS Style

    Ting Lei Zhao; Zhe Zhang; Dan Li; Yanxialei Jiang. Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles. Int. J. Biomed. Sci. Eng. 2022, 10(2), 54-60. doi: 10.11648/j.ijbse.20221002.14

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    AMA Style

    Ting Lei Zhao, Zhe Zhang, Dan Li, Yanxialei Jiang. Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles. Int J Biomed Sci Eng. 2022;10(2):54-60. doi: 10.11648/j.ijbse.20221002.14

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  • @article{10.11648/j.ijbse.20221002.14,
      author = {Ting Lei Zhao and Zhe Zhang and Dan Li and Yanxialei Jiang},
      title = {Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles},
      journal = {International Journal of Biomedical Science and Engineering},
      volume = {10},
      number = {2},
      pages = {54-60},
      doi = {10.11648/j.ijbse.20221002.14},
      url = {https://doi.org/10.11648/j.ijbse.20221002.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20221002.14},
      abstract = {Gold nanoparticles (AuNPs) have been widely studied in a great mass of cellular biomarkers detection and diagnostics due to their unique combination of physical and optical properties. Sea urchin-like gold nanoparticles (SUL-AuNPs) are well characterized multi-thorn gold nanostructures which possess at least four gold nanothorns on spherical gold surface, mimicking the morphology of sea urchin. Compared to spherical AuNPs, SUL-AuNPs showed a wide variety of light absorption and scattering properties, and the Surface-enhanced Raman Scattering (SERS) properties of SUL-AuNPs were also widely studied dependent on their surface morphology. Herein, three different diameters of SUL-AuNPs based on spherical AuNPs seed-mediated growth method by altering the amount of AuNPs in reaction system had been synthesized. The UV-vis spectrum of synthesized SUL-AuNPs displayed a shift from 550 nm to 650 nm compared to spherical AuNPs. FRET method was applied for the detection of GSH in hepatocytes and cell extracts using rhodamine B (RB) functionalized SUL-AuNPs, among the synthesized three different diameters SUL-AuNPs, 100nm RB-SUL-AuNPs displayed highest sensitivity for GSH detection. What's more, all synthesized SUL-AuNPs turned out to be membrane-permeable, and displayed ignorable cytotoxicity, which make SUL-AuNPs promising cellular thiols detection probes. In particular, it should be noted that the application of RB functionalized SUL-AuNPs exemplify ongoing efforts in design and utility of multifunctional nanoplatforms of SUL-AuNPs.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Cellular Detection of Glutathione Using Synthesized Stable Sea Urchin-Like Gold Nanoparticles
    AU  - Ting Lei Zhao
    AU  - Zhe Zhang
    AU  - Dan Li
    AU  - Yanxialei Jiang
    Y1  - 2022/06/27
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ijbse.20221002.14
    DO  - 10.11648/j.ijbse.20221002.14
    T2  - International Journal of Biomedical Science and Engineering
    JF  - International Journal of Biomedical Science and Engineering
    JO  - International Journal of Biomedical Science and Engineering
    SP  - 54
    EP  - 60
    PB  - Science Publishing Group
    SN  - 2376-7235
    UR  - https://doi.org/10.11648/j.ijbse.20221002.14
    AB  - Gold nanoparticles (AuNPs) have been widely studied in a great mass of cellular biomarkers detection and diagnostics due to their unique combination of physical and optical properties. Sea urchin-like gold nanoparticles (SUL-AuNPs) are well characterized multi-thorn gold nanostructures which possess at least four gold nanothorns on spherical gold surface, mimicking the morphology of sea urchin. Compared to spherical AuNPs, SUL-AuNPs showed a wide variety of light absorption and scattering properties, and the Surface-enhanced Raman Scattering (SERS) properties of SUL-AuNPs were also widely studied dependent on their surface morphology. Herein, three different diameters of SUL-AuNPs based on spherical AuNPs seed-mediated growth method by altering the amount of AuNPs in reaction system had been synthesized. The UV-vis spectrum of synthesized SUL-AuNPs displayed a shift from 550 nm to 650 nm compared to spherical AuNPs. FRET method was applied for the detection of GSH in hepatocytes and cell extracts using rhodamine B (RB) functionalized SUL-AuNPs, among the synthesized three different diameters SUL-AuNPs, 100nm RB-SUL-AuNPs displayed highest sensitivity for GSH detection. What's more, all synthesized SUL-AuNPs turned out to be membrane-permeable, and displayed ignorable cytotoxicity, which make SUL-AuNPs promising cellular thiols detection probes. In particular, it should be noted that the application of RB functionalized SUL-AuNPs exemplify ongoing efforts in design and utility of multifunctional nanoplatforms of SUL-AuNPs.
    VL  - 10
    IS  - 2
    ER  - 

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Author Information
  • College of Chemistry and Chemical Engineering, Linyi University, Linyi, China

  • College of Chemistry and Chemical Engineering, Jinan University, Jinan, China

  • College of Chemistry and Chemical Engineering, Linyi University, Linyi, China

  • College of Chemistry and Chemical Engineering, Linyi University, Linyi, China

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