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Identification and Application of Drosophila Novel Bioactive Peptides dRYamides

Received: 25 March 2015     Accepted: 2 April 2015     Published: 6 May 2015
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Abstract

The ligands of many orphan G protein-coupled receptors (GPCRs) remain to be identified, in both vertebrates and invertebrates, such as Drosophila melanogaster. Identification of their cognate ligands is critical for understanding the function and regulation of such GPCRs. Indeed, the discovery of bioactive peptides that bind GPCRs has enhanced our understanding of the mechanisms underlying many physiological processes. Here, we identified five endogenous ligands of the Drosophila orphan GPCRs, using functional assays and reverse pharmacological techniques. dRYamide-1 and -2 were found to be paired with the Drosophila neuropeptide Y (NPY)-like receptor (CG5811). Both dRYamide-1 and -2 contain a C-terminal RYamide. In vertebrates, RYamide motifs are found in NPY-family peptides. dRYamides were found to modulate feeding motivation in flies. These results suggest that deorphanizing the Drosophila orphan GPCRs might facilitate the elucidation of various physiological functions and identification of the ligands of orphan GPCRs in mammals.

Published in American Journal of Life Sciences (Volume 3, Issue 3-2)

This article belongs to the Special Issue Biology and Medicine of Peptide and Steroid Hormones

DOI 10.11648/j.ajls.s.2015030302.12
Page(s) 3-7
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Novel Bioactive Peptide, Orphan GPCR, Drosophila

References
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Cite This Article
  • APA Style

    Takanori Ida, Eri Iwamoto, Takahiro Sato, Masayasu Kojima. (2015). Identification and Application of Drosophila Novel Bioactive Peptides dRYamides. American Journal of Life Sciences, 3(3-2), 3-7. https://doi.org/10.11648/j.ajls.s.2015030302.12

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    ACS Style

    Takanori Ida; Eri Iwamoto; Takahiro Sato; Masayasu Kojima. Identification and Application of Drosophila Novel Bioactive Peptides dRYamides. Am. J. Life Sci. 2015, 3(3-2), 3-7. doi: 10.11648/j.ajls.s.2015030302.12

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    AMA Style

    Takanori Ida, Eri Iwamoto, Takahiro Sato, Masayasu Kojima. Identification and Application of Drosophila Novel Bioactive Peptides dRYamides. Am J Life Sci. 2015;3(3-2):3-7. doi: 10.11648/j.ajls.s.2015030302.12

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  • @article{10.11648/j.ajls.s.2015030302.12,
      author = {Takanori Ida and Eri Iwamoto and Takahiro Sato and Masayasu Kojima},
      title = {Identification and Application of Drosophila Novel Bioactive Peptides dRYamides},
      journal = {American Journal of Life Sciences},
      volume = {3},
      number = {3-2},
      pages = {3-7},
      doi = {10.11648/j.ajls.s.2015030302.12},
      url = {https://doi.org/10.11648/j.ajls.s.2015030302.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2015030302.12},
      abstract = {The ligands of many orphan G protein-coupled receptors (GPCRs) remain to be identified, in both vertebrates and invertebrates, such as Drosophila melanogaster. Identification of their cognate ligands is critical for understanding the function and regulation of such GPCRs. Indeed, the discovery of bioactive peptides that bind GPCRs has enhanced our understanding of the mechanisms underlying many physiological processes. Here, we identified five endogenous ligands of the Drosophila orphan GPCRs, using functional assays and reverse pharmacological techniques. dRYamide-1 and -2 were found to be paired with the Drosophila neuropeptide Y (NPY)-like receptor (CG5811). Both dRYamide-1 and -2 contain a C-terminal RYamide. In vertebrates, RYamide motifs are found in NPY-family peptides. dRYamides were found to modulate feeding motivation in flies. These results suggest that deorphanizing the Drosophila orphan GPCRs might facilitate the elucidation of various physiological functions and identification of the ligands of orphan GPCRs in mammals.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Identification and Application of Drosophila Novel Bioactive Peptides dRYamides
    AU  - Takanori Ida
    AU  - Eri Iwamoto
    AU  - Takahiro Sato
    AU  - Masayasu Kojima
    Y1  - 2015/05/06
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajls.s.2015030302.12
    DO  - 10.11648/j.ajls.s.2015030302.12
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 3
    EP  - 7
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.s.2015030302.12
    AB  - The ligands of many orphan G protein-coupled receptors (GPCRs) remain to be identified, in both vertebrates and invertebrates, such as Drosophila melanogaster. Identification of their cognate ligands is critical for understanding the function and regulation of such GPCRs. Indeed, the discovery of bioactive peptides that bind GPCRs has enhanced our understanding of the mechanisms underlying many physiological processes. Here, we identified five endogenous ligands of the Drosophila orphan GPCRs, using functional assays and reverse pharmacological techniques. dRYamide-1 and -2 were found to be paired with the Drosophila neuropeptide Y (NPY)-like receptor (CG5811). Both dRYamide-1 and -2 contain a C-terminal RYamide. In vertebrates, RYamide motifs are found in NPY-family peptides. dRYamides were found to modulate feeding motivation in flies. These results suggest that deorphanizing the Drosophila orphan GPCRs might facilitate the elucidation of various physiological functions and identification of the ligands of orphan GPCRs in mammals.
    VL  - 3
    IS  - 3-2
    ER  - 

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Author Information
  • Department of Bioactive Peptides, Frontier Science Research Center University of Miyazaki, Miyazaki, Japan

  • Department of Bioactive Peptides, Frontier Science Research Center University of Miyazaki, Miyazaki, Japan

  • Institute of Life Sciences, Kurume University, Fukuoka, Japan

  • Institute of Life Sciences, Kurume University, Fukuoka, Japan

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